Sperm parameters and oxidative stress markers in normal semen and abnormal semen

Abstract

Background: This study aimed to evaluate semen parameters which include sperm concentration, normal sperm morphology, progressive motility and semen volume in both normal and abnormal semen sample of infertility patients, as well as oxidative stress and antioxidants level in seminal plasma in normal and abnormal semen sample of infertility patients. Methods: Normal semen samples were collected from 41 infertile men and abnormal semen samples were collected from 47 infertile men and semen parameters and levels of total antioxidant capacity, nitric oxide, 8-hydroxydesoxyguanosine were examined in semen of 88 sample. Results: Seminal fluid analysis of specimen showed a significant difference (p < 0.05) in normal sperm morphology, sperm concentration, progressive motility and round cells and PH in abnormal semen group compared with the group of normozoospermia. And the results demonstrated statistically no significant increase (P≤0.05) in 8-OHdG (84.80±68.382 ng/mL), statistically no significant decrease in NO levels and TAC in the seminal plasma (0.033±0.019, 0.068±0.013 respectively) in Abnormal semen groups compared with normozoospermia. Conclusion: A significant difference (p < 0.05) in sperm concentration, round cells, progressive motility and PH, Normal sperm morphology in abnormal semen group compared with the group of normozoospermia. Non significant difference of oxidative stress in normal and abnormal semen sample of infertility patients.