Anti-inflammatory and anti-necrotic role of Brassica oleracea on pancreatic tissue exposed to toxic dose of paracetamol among rats.

Abstract

Background: Paracetamol is widely used as a pain reliever and antipyretic, and overdose may cause liver toxicity and pancreatic damage as a result of oxidative stress. Broccoli (Brassica oleracea), a member of the cruciferous family, contains biologically active compounds with strong antioxidant activity.   This s study aimed to evaluate the adequacy of alcoholic extract of broccoli against pancreatitis- induced with paracetamol Methodology : This study was conducted in the College of Education for Girls / University of Kufa, from the period 1/3/2023 to 1/5/2023, by following up on albino 40 male rats at the age of three months, , as the weight of the rats ranged between 216-246, were divided into four groups (each of 10 rats), the first group is the control group given tap water orally, the second group is treated with paracetamol drug concentration (2000 mg/kg) by mouth, and the third group is given the alcoholic extract of Brassica oleracea  (400 mg/kg) and paracetamol (2000 mg/kg) by mouth,  as for the last group was treated with broccoli extract (Brassica oleracea) of concentration (400 mg/kg) by mouth, and the dose continues for (30 days), once a day and now at eight o'clock.  This study indicated that the microscopic examination of the histological section of the pancreas. Results: Microscopic examination of this study showed observed pathological changes in the drug group, represented by vacuolation, acini cells necrosis, Langerhans islet distorted, vascular congestion, and lymph hyperplasia, and on the other hand, there were no clear pathological effects in the pancreatic tissues of the group that was treated with the drug and alcoholic extract, as well as the group that administered the extract only showed healthy pancreatic tissues. Conclusions:  From this, we conclude that the protective role of the broccoli plant against many of the pathological effects of paracetamol may be due to the good chemical content of antioxidants.