Genotyping of Invasive Pulmonary Aspergillosis through analysis of ITS rDNA sequences

Abstract

Aspergillus spores generally enter the lung gradually; nevertheless, due to human immunity, the spores cause a range of infectious, allergy, and respiratory diseases. Aspergillosis is more common in people who have severe immunodeficiency. The purpose of this study is to genotype Aspergillus species recovered from bronchoalveolar lavage (BAL) specimens. Lavage samples were cultivated on Sabouraud Dextrose Agar SDA, and a small piece of mycelia was subjected to DNA extraction, Synthesis and sequencing of Internal transcribed Spaser ITS, bioinformatics analysis, and Aspergillus species genotyping. In Lung Lavage, novel strains of A. terreus, A. sydowii, A. niger, and A. tubingensis were discovered. Many homozygous and heterozygous mutations were common frequently in the same site of sequence. It has been discovered that several genotypes were displayed in Aspergillus species based on sequencing comparison with reference strains stored in Genbank, and the pulmonary Aspergillosis with A.sydowii, A.terreus, A.tubingensis, and A.niger have been reported for the first time.