Karyotyping Analysis of Non-Iatrogenic, Non-Obstructive Azoospermic Males and Their Association with Sperm Retrieval ICSI Outcomes Among Iraqis

Abstract

Background: Recently, genetic analysis of male infertility gained specific attention, and there are only limited reports that have focused on azoospermic chromosomal aberrations and their association with reproductive future. This study intended to analyze the chromosomal aberrations of azoospermic patients and their association with the outcomes of ICSI post-sperm retrieval.

Methods: 76 azoospermic patients were counted in this cross-sectional study. All applicants were subjected to TESA, and both the positive and negative TESA underwent karyotyping. Only positive TESA results had been selected for further ICSI, whether they revealed normal or abnormal cytogenetic karyotyping.

Results:89.5% of patients presented with primary infertility. The abnormal karyotype represents 43.4% of patients. 63.7% of patients presented with Del y abnormality. The results in 68.4% of the total (52) infertile males who underwent TESA were presented with positive TESA results in 29 (55.8%). The majority of patients with positive TESA results had a normal karyotype. All those with positive TESA results underwent ICSI, and 14 out of the completed (82) ICSI cycles achieved positive clinical gestations, leading to the delivery of 7 babies, 4 processing, and 3 aborted fetuses. 57.2% of patients with ICSI had a normal karyotype. There was a non-significant association between karyotyping and the TESA results (P=0.6).

Conclusion

In NINOA, the majority of infertile males who presented with positive TESE results had normal karyotyping. There was no significant association between Karyotype with TESE and ICSI outcomes.